CATDOLL : CATDOLL: Reasonable use of fish oxytocin?

CATDOLL: Reasonable use of fish oxytocin?

1. How to use fish oxytocin reasonably?

The drugs currently used to induce spawning using dried fish include: the commonly used fish ovulation induction No. 2 (LHRH-A2), fish chorionic gonadotropin (polysexual hormone, HCG), chorionic gonadotropin type A, chorionic gonadotropin type B, fish ovulation induction No. 3 (LHRH-A3), diometron (DOM), fish pituitary gland (pituitary gland, PG) and a variety of complex high-efficiency oxytocin, but the most commonly used ones are fish ovulation induction No. 2 (LHRH-A2) and fish chorionic gonadotropin (polysexual hormone, HCG).

For fish with low spawning rate and low total spawning volume, such as loach, Chinese spiny target (Qingbo) and other medium and high-end fish, dioic acid (DOM) and pituitary gland (PG) should be added on the basis of using LHRH-A2+HCG, and the effect will be doubled.

For common fish, such as grass carp, silver carp, bighead carp, carp, crucian carp, etc., LHRH-A2+HCG is effective, and there is no need to add other oxytocin drugs to reduce production costs. Grass carp using LHRH-A2 alone, silver carp using chorionic gonadotropin type A alone, and bighead carp using chorionic gonadotropin type B alone are sufficient to achieve the purpose of smooth spawning. Also refer to: Rational use of fish oxytocin

2. What are the commonly used fish oxytocins?

Commonly used fish oxytocin agents include the following: 1. Oxygen: Oxygen is a hormone-based oxytocin agent, which is often used to increase the spawning rate of female fish and promote the maturation of follicles. Common oxytocin agents include synthetic GnRH (growth hormone-releasing hormone), hCG (human chorionic gonadotropin), etc. 2. Clonidine: Clonidine is an anesthetic, which is often used in the process of fish oxytocin. It can inhibit the physiological response of fish, reduce the stress response during spawning, and increase the spawning rate. 3. Ovaprim: Ovaprim is a commercial oxytocin agent made of a mixture of GnRH and hCG. It is often used in artificial breeding of marine fish and can effectively promote follicle maturation and spawning. 4. Acacetin: Acacetin is a plant extract with an oxytocin effect. It is often used in artificial breeding of freshwater fish and can increase the spawning rate of female fish and promote the maturation of follicles. It should be noted that the use of fish oxytocin agents should comply with relevant laws, regulations and technical specifications to avoid adverse effects on the environment and fish. At the same time, the use of oxytocin requires strict adherence to the instructions to ensure the effectiveness of oxytocin and the health and safety of the fish.

3. How to choose and use fish oxytocin?

Commonly used oxytocin hormones include: carp and crucian carp pituitary gland (PG), human chorionic gonadotropin (HCG), luteinizing hormone releasing hormone analogue (LRH-A), dioestrogen (DOM), reserpine (RES), etc.

The dosage is (calculated based on the weight of female fish, half for male fish): 1. Use fish pituitary alone: ​​⑴ One injection: 3-4 mg/kg for silver carp, grass carp, and bighead carp; 4-6 mg/kg for black carp; ⑵ Two injections: 0.3 mg/kg for the first injection of several fish, 3-4 mg for silver carp, grass carp, and bighead carp, and 4-6 mg for black carp. 2. Use HCG alone to induce labor in silver carp and bighead carp, with a single injection of 800-1200 international units (iu)/kg. 3. Use LRH-A alone to induce labor in grass carp: ⑴ One injection: 10-15 micrograms/kg; ⑵ Two injections: 1 microgram for the first injection and 10-15 micrograms for the second injection. 4. Mixed use of HCG and LRH-A: ⑴ Inducing spawning of silver carp and bighead carp: the first injection: 1-2 micrograms of LRH-A/kg; the second injection: 10-15 micrograms of LRH-A plus 300-400iuHCG/kg. ⑵ Inducing spawning of black carp: the first injection: 1-2 micrograms of LRH-A/kg; the second injection: 15-20 micrograms of LRH-A plus 800-1200iuHCG/kg. In recent years, some units have used high-efficiency new fish spawning mixtures, which have better breeding effects.

4. What is the basic principle of fish oxytocin?

The principle is to use a combination of physiological and ecological methods to directly inject oxytocin (PG, HCG or LRH-A, etc.) into the fish, replacing the effect of the gonadotropin secreted by the fish's own pituitary gland, or replacing the effect of the hypothalamus releasing LRH, which triggers the pituitary gland to secrete gonadotropin, thereby promoting oocyte maturation and spawning. Among fish oxytocin, the main components of the pituitary gland and HCG are FSH and LH. After entering the blood circulation of fish, they directly act on the gonads of carp, promoting gonadal maturation and spawning;

5. What is the oxytocin that cannot be used alone in fish farming?

1. How to use two types of fish oxytocin

1. Human chorionic gonadotropin (HCG) is one of the main oxytocin drugs used in artificial breeding of silver carp and bighead carp fry. The finished product is a white or light yellow powder, which is easily soluble in water and presents a colorless or light yellow clear liquid. Its aqueous solution is difficult to store and the storage time is generally less than 24 hours. It is easily ineffective when exposed to heat. Therefore, the product should be sealed and stored in a cool, dry place or refrigerator.

Usage (1) Dosage: Calculated per kilogram of broodstock, the injection amount for female fish is 800~1200 international units, and for male fish it is half. If used in combination with LRH-A, the effect is better. (2) Preparation of hormone injection: First prepare physiological saline (8 grams of sodium chloride and 1000 ml of distilled water or cold boiled water to fully dissolve). Boil the syringe and needle before use to disinfect. When using, use a syringe to draw a certain amount of physiological saline, insert the needle into the rubber stopper of the hormone bottle, inject the physiological saline, shake for 2~3 minutes, and after the hormone is fully dissolved, suck it out for use.

6. What is the "catalyst" needed for information warfare?

Reference answer: (The need for military struggle) is the "catalyst" for the formation of information warfare.

7. How to choose the stripping agent?

1. Selectivity of extractant and selectivity coefficient The selectivity of extractant refers to the difference in the solubility of extractant S for two components in the raw liquid. If the solubility of S for solute A is much greater than that for original solvent B, that is, the ratio in the extract phase is much greater and the ratio in the raffinate phase is much greater, then the selectivity of this extractant is good. The higher the selectivity of the extractant, the less the amount of extractant required to complete a certain separation task, and the corresponding energy consumption for solvent recovery operation is lower.

2. Mutual solubility of original solvent B and extractant S

3. Ease and economy of extractant recovery The E phase and R phase after extraction are usually separated by distillation. The ease of extractant recovery directly affects the cost of the extraction operation, thus largely determining the economy of the extraction process.

8. How to choose desiccant?

Desiccant, also called adsorbent, is used in moisture and mildew prevention to play a drying role. According to the adsorption method and reaction products, it can be divided into physical adsorption desiccant and chemical adsorption desiccant.

Physical adsorption desiccants include silica gel, alumina gel, molecular sieves, activated carbon, bone charcoal, charcoal, mineral desiccants, or activated white clay. Its drying principle is to physically adsorb water molecules into its own structure.

9. What are the principles for selecting extractants?

Extractant is another solvent that extracts solutes from one solution. It is used to extract solutes from different solutions, using the principle that the solubility of the solute in the two solutions is quite different.

Principles for selecting extraction solvents:

(1) “Like dissolves like”: the solubility of the active ingredient is high, while the solubility of the impurities is low.

(2) The solvent cannot affect the leached components. The solvent does not chemically react with the dissolved components, and even if it does, it is reversible.

(3) Cheap, readily available, safe to use, and easy to recycle and concentrate.

When solvents are used to extract drugs and their metabolites, alkaline drugs are extracted at an alkaline pH.

10. Why is a larger angle chosen for finishing and a smaller angle chosen for roughing?

Rough machining uses a small angle for rapid increments, while fine machining uses a large angle for cutting accuracy.

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